Download e-book for iPad: Advanced Photon Counting: Applications, Methods, by Peter Kapusta,Michael Wahl,Rainer Erdmann

By Peter Kapusta,Michael Wahl,Rainer Erdmann

This quantity makes a speciality of Time-Correlated unmarried Photon Counting (TCSPC), a strong software permitting luminescence lifetime measurements to be made with excessive temporal solution, even on unmarried molecules. Combining spectrum and lifelong presents a “fingerprint” for choosing such molecules within the presence of a historical past. Used including confocal detection, this allows single-molecule spectroscopy and microscopy as well as ensemble measurements, commencing up a major variety of sizzling existence technological know-how functions resembling fluorescence lifetime imaging (FLIM) and dimension of Förster Resonant power move (FRET) for the research of protein folding and interplay. a number of technology-related chapters current either the fundamentals and present cutting-edge, particularly of TCSPC electronics, photon detectors and lasers. the remainder chapters conceal a vast variety of purposes and methodologies for experiments and information research, together with the existence sciences, disorder facilities in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new recommendations bobbing up from the mix of vintage TCSPC and fluorescence lifetime with equipment in accordance with depth fluctuation characterize a very precise highlight.

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Advanced Photon Counting: Applications, Methods, Instrumentation (Springer Series on Fluorescence) by Peter Kapusta,Michael Wahl,Rainer Erdmann

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